Background: Human adipose-derived stem cells (hADSCs) are capable of differentiating\ninto many cells such as cardiac cells. Different types of inducers are used for cardiac\ncell differentiation, but this question still remains to be investigated, which one is the\nbest. The aim of this paper was to investigate the effect of combination of fibrin scaffold\nand trichostatin A (TSA), for differentiation of hADSCs into cardiomyocyte-like cells.\nMethods: After approval of characteristics of hADSCs and fibrin scaffold, hADSCs were\ncultured in fibrin scaffold with 10 �¼M TSA for 72 h and kept in standard conditions\nfor 4 weeks. QRT-PCR and immunostaining assay were performed for evaluating the\nexpression pattern of special cardiac genes and proteins.\nResults: In particular, our study showed that fibrin scaffold alongside TSA enhanced\nexpression of the selected genes and proteins.\nConclusions: We concluded that the TSA alone or with fibrin scaffold can lead to the\ngeneration of cardiac like cells in a short period of time.
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